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TNFa/Fas mediated apoptosis
1. transfect empty vector and p43 DNA(1ug;12well, 2ug;6 well) into target cells and incubate for 20 hrs at 5% Co2, 37 degree.
2. change with serum free media and add apoptotic mix(10ng of TNFa + 10ug/ml of cycloheximide or 1/1000 of Fax + 10ug of cycloheximide in serum free media).
3. culture for 1-2 hrs and watch morphological change & make a photo.
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