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Ponceau S staining 2005.2.2 Arthur
10X stock solution
2g Ponceau S, 30ml acetic acid, 70ml DW.
Protect the solution from light
Working solution
Dilute 10X solution with DW to 1X
Staining
After transfer you gel to PVDF membrane, briefly wash the membrane with DW, and stain the membrane with working Ponceau S solution for 2-5 min with gentle shaking. After confirm your protein, destain the membrane with DW till the membrane will be fully destained. Now you can go to the western procedure…
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