[안영하] Mouse_sample_preparation_for_western

This topic has 0 replies, 1 voice, and was last updated 16 years, 7 months ago by Seung Jae Jeong.

  • Author
    Posts
    • #19009

      Seung Jae Jeong
      Participant

       

      Mouse sample preparation for western

              1/31/01 by young-ha

       

       

      No-fix.

      Eppendorf tube,ice 준비.

      Sampling해서 ice에 박은 후 -200C이하에 storage.

       

      1. blood 체취 0.6~0.8ml정도의 blood 뽑는다.

      -40C에 하루정도 두면 위에 노랗게 분리되어

      나오는게 serum이다. 이것을 분리하여 -200C storage

      2.     모든 tissue 떼어내어 eppendorf tube 넣은 다음,

      ice 박는다.-200C이하에 storage.

      Thyroid , Salivary gland , Thymus , Heart , Lung , Liver , Spleen,

       

      Pancrease , Dueodenom , Small intestine , Large intestine

      Rectumn , Kidney , Bladder , Adrenal gland , Lymph node

      Ovary , Artery , Brain (대뇌,소뇌(cereberum), 연수)

      Pituitary gland

Viewing 0 reply threads

You must be logged in to reply to this topic.

CONTACT US

We're not around right now. But you can send us an email and we'll get back to you, asap.

Sending

©2010-2024 Medicinal Bioconvergence Research Center. All rights reserved.

Log in with your credentials

Forgot your details?