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C2C12 differentiation into myocyte
1. Seed the cells into 12 well plate and culture the cells with DMEM containing 10% FBS
2. Culture till confluent and culture the cells 2 days more at the confluent status
3. Change the media with DMEM containing 2% horse serum and culture for 2 days
4. Change the media at every 2 day and culture for 2 days
5. Repeat step 4 till differentiation rate of C2C12 cells is 80% è you can see the differentiation rate by examination of your cells under the light microscope
è cell are differentiated, you can see the multinucleated myotube
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