[cell biology work] growth_curve_of_MEF

[Seung Jae Jeong]

 

                                                        9.29.20 from Cell. Vol.91. 25-34 by LHW

                                                          3T3 protocol (Todaro and Green, 1963)

< Growth Curve of MEF cells >

1.     MEF cells prep.

2.     plating 10⁶ cells on a 10cm dish.

3.     3 days incubation.

4.     cell counting and replating 10⁶ cells

 

 

 

 

– population doubling number –

 

PDL = log(n_f/n₀)/log2

  n₀ : the initial number of cells

n_f : the final number of cells

 

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