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20X SSC
20% SDS
D.W.
2X SSC Sol.
10ml
0.5ml
89.5ml
1X SSC Sol.
5ml
0.5ml
94.5ml
0.1X SSC Sol.
0.5ml
0.5ml
99.0ml
Washing Solution(100ml기준)
Hybridization Solution
N.H. 20ml
S.H.
50% formamide
S.H.
30% formamide
50% formamide
30% formamide
30ml (prehyb.)
20ml (hyb.)
20ml (prehyb.)
5ml (hyb.)
Formamide
10
6
15
10
6
1.5
100Xdenhardt’s
1
1
1.5
1
1
0.25
20X SSC
5
5
7.5
5
5
1.25
20% SDS
0.1
0.1
1.5
1
1
0.25
SsDNA
0.2
0.2
0.2
0
0.2
0
D.W.
3.7
7.7
1.3
1
4.8
1.25
50%Dextransulf
3
2
2
0.5
MicroArray
1. p43 F, N, C / THP-1 / 2hr
Con.
p43 F
p43 N
p43 C
p43N+C
2. 293-Q, 293-3 HEK293
293-Q
293-3
3. p43 2hr / Cycloheximide 1hr / THP-1
Con.
p43F
cycloheximide
,p43+cycloheximide
4. p38 transfection / HEK293
Con(EV)
p38transfection
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