[northern] 20X SSC

20X SSC

20% SDS

D.W.

2X SSC Sol.

10ml

0.5ml

89.5ml

1X SSC Sol.

5ml

0.5ml

94.5ml

0.1X SSC Sol.

0.5ml

0.5ml

99.0ml

N.H. 20ml

S.H.

50% formamide

S.H.

30% formamide

50% formamide

30% formamide

30ml (prehyb.)

20ml (hyb.)

20ml (prehyb.)

5ml (hyb.)

Formamide

10

6

15

10

6

1.5

100Xdenhardt’s

1

1

1.5

1

1

0.25

20X SSC

5

5

7.5

5

5

1.25

20% SDS

0.1

0.1

1.5

1

1

0.25

SsDNA

0.2

0.2

0.2

0

0.2

0

D.W.

3.7

7.7

1.3

1

4.8

1.25

50%Dextransulf

3

2

2

0.5

1. p43 F, N, C  / THP-1  /  2hr

Con.

p43 F

p43 N

p43 C

p43N+C

2. 293-Q, 293-3   HEK293

293-Q

293-3

3. p43 2hr / Cycloheximide 1hr / THP-1

Con.

p43F

cycloheximide

,p43+cycloheximide

4. p38 transfection /  HEK293

Con(EV)

p38transfection