[cell biology work] SA_β-gal_staining_in_fibroblasts

SA β-gal staining (in fibroblasts)     Ref. PNAS95-92-9363

1.     culture cells (up to 70% confluency)

2.     washing with 1xPBS briefly   X  2ea

3.     fix with Fixing Sol. 5min at R.T.

working            stock        1ml          5ml       10ml  

37% formaldehyde    2%        55ul         275ul       550ul

25% glutaraldehyde   0.2%       8ul          40ul       80ul

   
1X PBS                               937ul    4.685ml      9.37ml

4. washing with 1XPBS 5min   X   3ea

      5. staining with SA- β-gal staining Sol. 2hr – 16hr at 37℃ (w/o CO₂)

working             stock

1mg/ml of X-gal        20mg/ml of DMFA

40mM Citric acid