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	<title>Biocon | Seung Jae Jeong | Activity</title>
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				<title>Seung Jae Jeong&#039;s profile was updated</title>
				<link>https://target.re.kr/activate/p/39450/</link>
				<pubDate>Wed, 16 May 2018 03:28:45 +0900</pubDate>

				
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				<title>Seung Jae Jeong&#039;s profile was updated</title>
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				<pubDate>Fri, 02 Mar 2018 10:06:46 +0900</pubDate>

				
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				<title>Seung Jae Jeong&#039;s profile was updated</title>
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				<pubDate>Tue, 19 Dec 2017 02:03:16 +0900</pubDate>

				
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				<title>Seung Jae Jeong joined the group BioBank</title>
				<link>https://target.re.kr/activate/p/39331/</link>
				<pubDate>Tue, 19 Dec 2017 01:35:18 +0900</pubDate>

				
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				<title>Seung Jae Jeong joined the group CellBio1</title>
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				<pubDate>Thu, 23 Jun 2016 04:36:32 +0900</pubDate>

				
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				<title>Seung Jae Jeong changed their profile picture</title>
				<link>https://target.re.kr/activate/p/188/</link>
				<pubDate>Thu, 23 Jun 2016 04:35:14 +0900</pubDate>

				
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				<title>Seung Jae Jeong started the topic [민유홍] RevTet-Off System Protocol in the forum Protocol</title>
				<link>https://biocon.re.kr/forums/topic/18939/</link>
				<pubDate>Mon, 02 Jun 2008 01:37:33 +0900</pubDate>

									<content:encoded><![CDATA[<p><span><b><span>RevTet-Off System Protocol</span></b><span>2007. 6.20</span><span> Yu-Hong Min</span><b><span>&nbsp;</span></b><b><span>Guidelines for working with retroviruses</span></b><span>Put on double gloves.</span><span>All waste must be autoclaved.</span><span>Liquid waste : Do not suction. Collect in glass bottle then autoclave.</span><span>&nbsp;</span><b><span>PT67 packaging cell culture</span></b><span>DMEM, 10% tetracycline-free FBS, 1% P/G</span><span>&nbsp;</span><b><span>Doxycycline</span></b><span>Dissolve at 1 mg/ml in water and filter sterilize. Store at&hellip;</span></span><span class="activity-read-more" id="activity-read-more-39551"><a href="https://biocon.re.kr/forums/topic/18939/" rel="nofollow">[Read more]</a></span></p>
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				<title>Seung Jae Jeong started the topic [mouse work] X-gal_staining_in_Embryo in the forum Protocol</title>
				<link>https://biocon.re.kr/forums/topic/18924/</link>
				<pubDate>Mon, 02 Jun 2008 01:22:18 +0900</pubDate>

									<content:encoded><![CDATA[<p><strong><span>1)<span>&nbsp; </span></span></strong><strong><span>On Frozen sections</span></strong><strong><span></span></strong><strong><span>a. X-Gal Staining of <span>Embryos frozen sections</span> or Tibia</span></strong><span></span><span>&nbsp;</span><span>Day 16.5/17.5 embryos were frozen on dry ice in tissue freezing medium (Leica) and cut into 15 µm sections. Tibiae were cut longitudinally. After fixation in 4% PFA for 5 min, specimen were incubated 24 hr in PBS containing 5 mM K3, 5 mM K4, 2 mM MgCl2, and 1mg/ml X-Gal&hellip;</span><span class="activity-read-more" id="activity-read-more-39555"><a href="https://biocon.re.kr/forums/topic/18924/" rel="nofollow">[Read more]</a></span></p>
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				<title>Seung Jae Jeong started the topic [molecular biology work] Bio Rad IEF cell protocol in the forum Protocol</title>
				<link>https://biocon.re.kr/forums/topic/18866/</link>
				<pubDate>Fri, 30 May 2008 06:45:19 +0900</pubDate>

									<content:encoded><![CDATA[<p><span>Bio Rad IEF cell(Model #PROTEAN IEF Cell) protocol</span><span><span>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; &nbsp;</span>Arthur</span><span>&nbsp;</span><span>&nbsp;</span><span>&nbsp;</span><span><span>1.<span> </span></span></span><span>spin down the cell at 1,500Xg for 5 min</span><span><span>2.<span> </span></span></span><span>wash the cell with ice cold PBS and lyse the cells with lysis buffer(1X PBS containing 1%&hellip;</span><span class="activity-read-more" id="activity-read-more-39557"><a href="https://biocon.re.kr/forums/topic/18866/" rel="nofollow">[Read more]</a></span></p>
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				<title>Seung Jae Jeong started the topic [molecular biology work] GST_pulldown_assay in the forum Protocol</title>
				<link>https://biocon.re.kr/forums/topic/18859/</link>
				<pubDate>Fri, 30 May 2008 06:42:07 +0900</pubDate>

									<content:encoded><![CDATA[<p><b><span>GST pull down assay</span></b><span>&nbsp;</span><b><span>A. Expression of GST fusion protein</span></b><span><span>&nbsp;</span></span><span><span>1.<span>&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><span>Inoculate 2ml of LB/antibiotics with 1 colony</span><span><span>2.<span>&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><span>O/N culture with shaking at 37</span><span>℃</span><span></span><span><span>3.<span>&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><span>Inoculate pre-warmed 25ml of LB/antibiotics with all of </span><span>2ml-cultrure</span><span><span>4.<span>&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><span>1 hr at 37</span><span>℃</span><span> with shaking vigorously</span><span><span>5.<span>&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><span>Inoculate pre-warmed 500 ml of LB/antibiotics with all of</span><span>25ml-culture</span><span><span>6.<span>&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span><span>Shak&hellip;</span><span class="activity-read-more" id="activity-read-more-39556"><a href="https://biocon.re.kr/forums/topic/18859/" rel="nofollow">[Read more]</a></span></p>
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